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Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brai...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Duguid, J R, Rohwer, R G, Seed, B
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281836/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2456582/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.15.5738
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