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tRNA Ligase Catalyzes the GTP-Dependent Ligation of RNA with 3′-Phosphate and 5′-Hydroxyl Termini

The RNA ligase RtcB is conserved in all domains of life, and is essential for tRNA maturation in archaea and metazoa. Here we show that bacterial and archaeal RtcB catalyze the GTP-dependent ligation of RNA with 3′-phosphate and 5′-hydroxyl termini. Reactions with analogues of RNA and GTP suggest a...

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Detalhes bibliográficos
Main Authors: Desai, Kevin K., Raines, Ronald T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3304435/
https://ncbi.nlm.nih.gov/pubmed/22320833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201921a
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