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A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis

Despite intensive research, there are very few reagents with which to modulate and dissect the mRNA splicing pathway. Here, we describe a novel approach to identify such tools, based on detection of the exon junction complex (EJC), a unique molecular signature that splicing leaves on mRNAs. We devel...

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Autori principali: Berg, Michael G., Wan, Lili, Younis, Ihab, Diem, Michael D., Soo, Michael, Wang, Congli, Dreyfuss, Gideon
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3302438/
https://ncbi.nlm.nih.gov/pubmed/22252314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.05788-11
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