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Single-pot enzymatic synthesis of Dicer-substrate siRNAs
We describe an inexpensive and efficient method for generating functional pools of Dicer-substrate small interfering RNAs (siRNAs) in a single reaction tube. The method exploits a highly active form of the enzyme Dicer from Giardia lamblia, which is capable of accurately processing double-stranded R...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3299999/ https://ncbi.nlm.nih.gov/pubmed/22189103 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1174 |
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