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Single-pot enzymatic synthesis of Dicer-substrate siRNAs

We describe an inexpensive and efficient method for generating functional pools of Dicer-substrate small interfering RNAs (siRNAs) in a single reaction tube. The method exploits a highly active form of the enzyme Dicer from Giardia lamblia, which is capable of accurately processing double-stranded R...

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Détails bibliographiques
Auteurs principaux: Guiley, Keelan Z., Pratt, Ashley J., MacRae, Ian J.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3299999/
https://ncbi.nlm.nih.gov/pubmed/22189103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1174
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