Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones.

The 264 bp mini-transposon Tn5supF was constructed to sequence DNAs cloned in phage lambda without extensive shotgun subcloning or primer walking. Unique sequences near each transposon end serve as primer binding sites, and a supF gene is used to select transposition to lambda. We describe here PCR...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Krishnan, B R, Kersulyte, D, Brikun, I, Berg, C M, Berg, D E
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1991
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC329118/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1659687/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.22.6177
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