Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.

We constructed a derivative of transposon Tn5 called Tn5supF for insertion mutagenesis and sequencing DNAs cloned in phage lambda. This element carries a supF amber-suppressor tRNA gene. Its insertion into lambda can be selected by plaque formation by using nonsuppressing (sup0) Escherichia coli for...

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Detalles Bibliográficos
Autores principales: Phadnis, S H, Huang, H V, Berg, D E
Formato: Artigo
Lenguaje:Inglês
Publicado: 1989
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC297740/
https://ncbi.nlm.nih.gov/pubmed/2548192
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