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Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.

We constructed a derivative of transposon Tn5 called Tn5supF for insertion mutagenesis and sequencing DNAs cloned in phage lambda. This element carries a supF amber-suppressor tRNA gene. Its insertion into lambda can be selected by plaque formation by using nonsuppressing (sup0) Escherichia coli for...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Phadnis, S H, Huang, H V, Berg, D E
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297740/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2548192/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5908
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