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Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones.
The 264 bp mini-transposon Tn5supF was constructed to sequence DNAs cloned in phage lambda without extensive shotgun subcloning or primer walking. Unique sequences near each transposon end serve as primer binding sites, and a supF gene is used to select transposition to lambda. We describe here PCR...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1991
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC329118/ https://ncbi.nlm.nih.gov/pubmed/1659687 |
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