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Bioengineering of coagulation factor VIII for efficient expression through elimination of a dispensable disulfide loop

BACKGROUND: Heterologous expression of Factor VIII (FVIII) is about 2 to 3 orders of magnitude lower than similarly sized proteins. Bioengineering strategies aimed at different structural and biochemical attributes of FVIII have been successful in enhancing its expression levels. OBJECTIVE: Disulfid...

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Autors principals: SELVARAJ, SUNDAR R, SCHELLER, ARNO N, MIAO, HONGZHI Z, KAUFMAN, RANDAL J, PIPE, STEVEN W
Format: Artigo
Idioma:Inglês
Publicat: 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290727/
https://ncbi.nlm.nih.gov/pubmed/22044596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1538-7836.2011.04545.x
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