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Bioengineering of coagulation factor VIII for efficient expression through elimination of a dispensable disulfide loop
BACKGROUND: Heterologous expression of Factor VIII (FVIII) is about 2 to 3 orders of magnitude lower than similarly sized proteins. Bioengineering strategies aimed at different structural and biochemical attributes of FVIII have been successful in enhancing its expression levels. OBJECTIVE: Disulfid...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3290727/ https://ncbi.nlm.nih.gov/pubmed/22044596 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1538-7836.2011.04545.x |
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