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A semisynthetic Eph receptor tyrosine kinase provides insight into ligand-induced kinase activation

We have developed a methodology for generating milligram amounts of functional Eph tyrosine kinase receptor using the protein engineering approach of expressed protein ligation. Stimulation with ligand induces efficient autophosphorylation of the semisynthetic Eph construct. The in vitro phosphoryla...

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Detalles Bibliográficos
Autores principales: Singla, Nikhil, Erdjument-Bromage, Hediye, Himanen, Juha P., Muir, Tom W., Nikolov, Dimitar B.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3286648/
https://ncbi.nlm.nih.gov/pubmed/21439481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.01.011
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