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A semisynthetic Eph receptor tyrosine kinase provides insight into ligand-induced kinase activation
We have developed a methodology for generating milligram amounts of functional Eph tyrosine kinase receptor using the protein engineering approach of expressed protein ligation. Stimulation with ligand induces efficient autophosphorylation of the semisynthetic Eph construct. The in vitro phosphoryla...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3286648/ https://ncbi.nlm.nih.gov/pubmed/21439481 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.01.011 |
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