Kinetics of tRNA folding monitored by aminoacylation

We describe a strategy for tracking Mg(2+)-initiated folding of (32)P-labeled tRNA molecules to their native structures based on the capacity for aminoacylation by the cognate aminoacyl-tRNA synthetase enzyme. The approach directly links folding to function, paralleling a common strategy used to stu...

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Bibliographic Details
Main Authors: Bhaskaran, Hari, Rodriguez-Hernandez, Annia, Perona, John J.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2012
Subjects:
Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3285943/
https://ncbi.nlm.nih.gov/pubmed/22286971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.030080.111
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