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Synthesis of Glu-tRNA(Gln) by engineered and natural aminoacyl-tRNA synthetases
A protein engineering approach is described to delineate which distinct elements of homologous tRNA synthetase architectures are responsible for divergent RNA-amino acid pairing specificities. Previously, we constructed a hybrid enzyme in which 23 amino acids from the catalytic domain of E. coli glu...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2921816/ https://ncbi.nlm.nih.gov/pubmed/20617848 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi100886z |
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