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A 5′-terminal phosphate is required for stable ternary complex formation and translation of leaderless mRNA in Escherichia coli
The bacteriophage λ's cI mRNA was utilized to examine the importance of the 5′-terminal phosphate on expression of leadered and leaderless mRNA in Escherichia coli. A hammerhead ribozyme was used to produce leadered and leaderless mRNAs, in vivo and in vitro, that contain a 5′-hydroxyl. Althoug...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cold Spring Harbor Laboratory Press
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3285938/ https://ncbi.nlm.nih.gov/pubmed/22291205 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.027698.111 |
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