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A 5′-terminal phosphate is required for stable ternary complex formation and translation of leaderless mRNA in Escherichia coli

The bacteriophage λ's cI mRNA was utilized to examine the importance of the 5′-terminal phosphate on expression of leadered and leaderless mRNA in Escherichia coli. A hammerhead ribozyme was used to produce leadered and leaderless mRNAs, in vivo and in vitro, that contain a 5′-hydroxyl. Althoug...

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Bibliografiske detaljer
Main Authors: Giliberti, Jacqueline, O'Donnell, Sean, Van Etten, William J., Janssen, Gary R.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3285938/
https://ncbi.nlm.nih.gov/pubmed/22291205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.027698.111
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