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A novel expression system for production of soluble prion proteins in E. coli

Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, the need to obtain large quantities of the recombinant protein for research pu...

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Main Authors: Abskharon, Romany NN, Ramboarina, Stephanie, El Hassan, Hassan, Gad, Wael, Apostol, Marcin I, Giachin, Gabriele, Legname, Giuseppe, Steyaert, Jan, Messens, Joris, Soror, Sameh H, Wohlkonig, Alexandre
格式: Artigo
語言:Inglês
出版: BioMed Central 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3283519/
https://ncbi.nlm.nih.gov/pubmed/22233534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-6
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