Overexpression, purification and crystallization of BamHI endonuclease.

The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which...

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Publicat a:Nucleic Acids Res
Autors principals: Jack, W E, Greenough, L, Dorner, L F, Xu, S Y, Strzelecka, T, Aggarwal, A K, Schildkraut, I
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1991
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328111/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2030964/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.8.1825
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