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Engineering Infrequent DNA Nicking Endonuclease by Fusion of a BamHI Cleavage-Deficient Mutant and a DNA Nicking Domain

Strand-specific DNA nicking endonucleases (NEases) typically nick 3–7 bp sites. Our goal is to engineer infrequent NEase with a >8 bp recognition sequence. A BamHI catalytic-deficient mutant D94N/E113K was constructed, purified, and shown to bind and protect the GGATCC site from BamHI restriction...

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מחבר ראשי: Shuang-yong Xu
פורמט: Artigo
שפה:Inglês
יצא לאור: Frontiers Media S.A. 2022-02-01
סדרה:Frontiers in Microbiology
נושאים:
BamHI catalytic mutant
phage Gamma HNH nicking domain
infrequent nicking enzyme
protein engineering
DNA nicking assay
גישה מקוונת:https://www.frontiersin.org/articles/10.3389/fmicb.2021.787073/full
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