Overexpression, purification and crystallization of BamHI endonuclease.
The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which...
Tallennettuna:
| Julkaisussa: | Nucleic Acids Res |
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| Päätekijät: | , , , , , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Oxford University Press
1991
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| Linkit: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2030964/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.8.1825 |
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