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Overexpression, purification and crystallization of BamHI endonuclease.
The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1991
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328111/ https://ncbi.nlm.nih.gov/pubmed/2030964 |
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