The synthesis of oligodeoxyribonucleotides using RNA ligase.
T4 RNA ligase catalyzes the addition of a single deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of oligodeoxyribonucleotides (Hinton et al. (1978) Biochemistry 17, 5091). We have determined improved conditions for this reaction which give yields equal to or greater than 85% when any of fi...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1979
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| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328028/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/386284/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/7.2.453 |
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