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The synthesis of oligodeoxyribonucleotides using RNA ligase.
T4 RNA ligase catalyzes the addition of a single deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of oligodeoxyribonucleotides (Hinton et al. (1978) Biochemistry 17, 5091). We have determined improved conditions for this reaction which give yields equal to or greater than 85%...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328028/ https://ncbi.nlm.nih.gov/pubmed/386284 |
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