The synthesis of oligodeoxyribonucleotides using RNA ligase.
T4 RNA ligase catalyzes the addition of a single deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of oligodeoxyribonucleotides (Hinton et al. (1978) Biochemistry 17, 5091). We have determined improved conditions for this reaction which give yields equal to or greater than 85% when any of fi...
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| Gepubliceerd in: | Nucleic Acids Res |
|---|---|
| Hoofdauteurs: | , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Oxford University Press
1979
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| Online toegang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328028/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/386284/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/7.2.453 |
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