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The synthesis of oligodeoxyribonucleotides using RNA ligase.

T4 RNA ligase catalyzes the addition of a single deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of oligodeoxyribonucleotides (Hinton et al. (1978) Biochemistry 17, 5091). We have determined improved conditions for this reaction which give yields equal to or greater than 85%...

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Bibliografiske detaljer
Main Authors: Hinton, D M, Gumport, R I
Format: Artigo
Sprog:Inglês
Udgivet: 1979
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC328028/
https://ncbi.nlm.nih.gov/pubmed/386284
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