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A role for a single-stranded junction in RNA binding and specificity by the Tetrahymena group I ribozyme

We have investigated the role of a single-stranded RNA junction, J1/2, that connects the substrate-containing P1 duplex to the remainder of the Tetrahymena group I ribozyme. Single turnover kinetics, fluorescence anisotropy, and single molecule FRET for a series of J1/2 mutants were used to probe th...

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Detalhes bibliográficos
Main Authors: Shi, Xuesong, Solomatin, Sergey V., Herschlag, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277301/
https://ncbi.nlm.nih.gov/pubmed/22220837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja2083575
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