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Extraordinarily slow binding of guanosine to the Tetrahymena group I ribozyme: Implications for RNA preorganization and function

The Tetrahymena ribozyme derived from the self-splicing group I intron binds a 5′-splice site analog (S) and guanosine (G), catalyzing their conversion to a 5′-exon analog (P) and GA. Herein, we show that binding of guanosine is exceptionally slow, limiting the reaction at near neutral pH. Our resul...

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Bibliografische gegevens
Hoofdauteurs: Karbstein, Katrin, Herschlag, Daniel
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2003
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC151335/
https://ncbi.nlm.nih.gov/pubmed/12591943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.252749799
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