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Extraordinarily slow binding of guanosine to the Tetrahymena group I ribozyme: Implications for RNA preorganization and function
The Tetrahymena ribozyme derived from the self-splicing group I intron binds a 5′-splice site analog (S) and guanosine (G), catalyzing their conversion to a 5′-exon analog (P) and GA. Herein, we show that binding of guanosine is exceptionally slow, limiting the reaction at near neutral pH. Our resul...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC151335/ https://ncbi.nlm.nih.gov/pubmed/12591943 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.252749799 |
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