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A method for the enzymatic synthesis and purification of [alpha-32P] nucleoside triphosphates.
A simplified method is described for the enzymatic synthesis and purification of [alpha-32P]ribo- and deoxyribonucleoside triphosphates. The products are obtained at greater than 97% radiochemical purity with yields of 50--70% (relative to 32Pi) by a two-step elution from DEAE-Sephadex. All reaction...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1979
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/218171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.1.81 |
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