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An approach to crystallizing proteins by metal-mediated synthetic symmetrization

Combining the concepts of synthetic symmetrization with the approach of engineering metal-binding sites, we have developed a new crystallization methodology termed metal-mediated synthetic symmetrization. In this method, pairs of histidine or cysteine mutations are introduced on the surface of targe...

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Hlavní autoři: Laganowsky, Arthur, Zhao, Minglei, Soriaga, Angela B, Sawaya, Michael R, Cascio, Duilio, Yeates, Todd O
Médium: Artigo
Jazyk:Inglês
Vydáno: Wiley Subscription Services, Inc., A Wiley Company 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3267952/
https://ncbi.nlm.nih.gov/pubmed/21898649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.727
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