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Competing allosteric mechanisms modulate substrate binding in a dimeric enzyme
Allostery has been studied for many decades yet it remains challenging to determine experimentally how it occurs at a molecular level. We have developed an approach combining isothermal titration calorimetry, circular dichroism, and nuclear magnetic resonance spectroscopy to quantify allostery in te...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3262843/ https://ncbi.nlm.nih.gov/pubmed/21278754 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.1978 |
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