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Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms

Isothermal titration calorimetry (ITC) is commonly used to determine the thermodynamic parameters associated with the binding of a ligand to a host macromolecule. ITC has some advantages over common spectroscopic approaches for studying host/ligand interactions. For example, the heat released or abs...

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Bibliografske podrobnosti
Main Authors: Freiburger, Lee A., Mittermaier, Anthony K., Auclair, Karine
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169262/
https://ncbi.nlm.nih.gov/pubmed/21505408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2529
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