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Directed Evolution of the Transcriptional Regulator DntR: Isolation of Mutants with Improved DNT-Response

The transcriptional regulator DntR, which previously has been isolated from bacterial strains capable of degrading 2,4-dinitrotoluene (DNT), was engineered in order to improve the ability to detect DNT. A directed evolution strategy was employed, where sequence diversity first was created by random...

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Detalhes bibliográficos
Main Authors: Lönneborg, Rosa, Varga, Edina, Brzezinski, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3261848/
https://ncbi.nlm.nih.gov/pubmed/22276138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0029994
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