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Directed evolution of the transcription factor XylS for development of improved expression systems
The inducible Pm promoter together with its cognate positive transcription regulator XylS has been shown to be useful for recombinant protein production under high cell density conditions. Here we report directed evolution of XylS resulting in mutant proteins with increased ability to stimulate tran...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Blackwell Publishing Ltd
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815945/ https://ncbi.nlm.nih.gov/pubmed/21255304 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2009.00126.x |
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