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The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning expe...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1983
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325756/ https://ncbi.nlm.nih.gov/pubmed/6300769 |
| タグ: |
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