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The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning expe...
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| Asıl Yazarlar: | , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
1983
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| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325756/ https://ncbi.nlm.nih.gov/pubmed/6300769 |
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