The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning expe...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1983
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325756/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6300769/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.3.837 |
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