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The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.

The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning expe...

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Détails bibliographiques
Auteurs principaux: Brooks, J E, Blumenthal, R M, Gingeras, T R
Format: Artigo
Langue:Inglês
Publié: 1983
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC325756/
https://ncbi.nlm.nih.gov/pubmed/6300769
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