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Efficient Digestion and Mass Spectral Analysis of Vesicular Glutamate Transporter 1: A Recombinant Membrane Protein Expressed in Yeast
Attempts to characterize recombinant integral membrane proteins (IMPs) by mass spectrometry are frequently hindered by several factors including the detergents required for extraction and purification that interferes with analysis, poor solubility, incomplete digestion, and limited identification of...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3247074/ https://ncbi.nlm.nih.gov/pubmed/18179165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr070452b |
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