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Efficient Digestion and Mass Spectral Analysis of Vesicular Glutamate Transporter 1: A Recombinant Membrane Protein Expressed in Yeast

Attempts to characterize recombinant integral membrane proteins (IMPs) by mass spectrometry are frequently hindered by several factors including the detergents required for extraction and purification that interferes with analysis, poor solubility, incomplete digestion, and limited identification of...

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Detalhes bibliográficos
Main Authors: Cox, Holly D., Chao, Chih-Kai, Patel, Sarjubhai A., Thompson, Charles M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3247074/
https://ncbi.nlm.nih.gov/pubmed/18179165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr070452b
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