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Evaluating the Catalytic Contribution from the Oxyanion Hole in Ketosteroid Isomerase
Prior site-directed mutagenesis studies in bacterial ketosteroid isomerase (KSI) reported that substitution of both oxyanion hole hydrogen bond donors give a 10(5)–10(8) fold rate reduction suggesting that the oxyanion hole may provide the major contribution to KSI catalysis. But these seemingly con...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3241876/ https://ncbi.nlm.nih.gov/pubmed/22053826 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja208050t |
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