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Evaluating the Catalytic Contribution from the Oxyanion Hole in Ketosteroid Isomerase

Prior site-directed mutagenesis studies in bacterial ketosteroid isomerase (KSI) reported that substitution of both oxyanion hole hydrogen bond donors give a 10(5)–10(8) fold rate reduction suggesting that the oxyanion hole may provide the major contribution to KSI catalysis. But these seemingly con...

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Bibliografiske detaljer
Main Authors: Schwans, Jason P., Sunden, Fanny, Gonzalez, Ana, Tsai, Yingssu, Herschlag, Daniel
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3241876/
https://ncbi.nlm.nih.gov/pubmed/22053826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja208050t
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