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Toward computer-aided site-directed mutagenesis of enzymes.

A preliminary attempt to simulate the observed effect of a site-directed mutagenesis of rat trypsin gives encouraging results. The calculations reproduce in a semiquantitative way the observed change in the activation barrier of the rate-limiting step of amide hydrolysis. This result, which did not...

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Main Authors: Warshel, A, Sussman, F
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC323612/
https://ncbi.nlm.nih.gov/pubmed/3520555
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