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Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.

We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the gene can be selected by growth in the presence of low concentrations of the ADA inhibitor 2'-deoxy...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Kaufman, R J, Murtha, P, Ingolia, D E, Yeung, C Y, Kellems, R E
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323467/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3486414/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.10.3136
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