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Translational efficiency of polycistronic mRNAs and their utilization to express heterologous genes in mammalian cells.

The translation of polycistronic mRNAs in mammalian cells was studied. Transcription units, constructed to contain one, two or three open reading frames (ORFs), were introduced stably into Chinese hamster ovary cells and transiently into COS monkey cells. The analysis of mRNA levels and protein synt...

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Detalhes bibliográficos
Main Authors: Kaufman, R J, Murtha, P, Davies, M V
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC553375/
https://ncbi.nlm.nih.gov/pubmed/3582359
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