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Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the gene can be selected by growth in the presence of low concentrations of the ADA inhibitor 2'-deoxy...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1986
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323467/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3486414/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.10.3136 |
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