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Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera

Dihydrodipicolinate synthase (DHDPS) catalyses the first committed step of the lysine-biosynthesis pathway in bacteria, plants and some fungi. This study describes the cloning, expression, purification and crystallization of DHDPS from the grapevine Vitis vinifera (Vv-DHDPS). Following in-drop cleav...

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Detalhes bibliográficos
Main Authors: Atkinson, Sarah C., Dogovski, Con, Newman, Janet, Dobson, Renwick C. J., Perugini, Matthew A.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232133/
https://ncbi.nlm.nih.gov/pubmed/22139160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111038395
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