Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila

Dihydrodipicolinate synthase (DHDPS) catalyses the rate-limiting step in the biosynthesis of meso-diaminopimelate and lysine. Here, the cloning, expression, purification and crystallization of DHDPS from the intracellular pathogen Legionella pneumophila are described. Crystals grown in the presence...

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Bibliographic Details
Main Authors: Siddiqui, Tanzeela, Paxman, Jason J., Dogovski, Con, Panjikar, Santosh, Perugini, Matthew A.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2013
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792684/
https://ncbi.nlm.nih.gov/pubmed/24100576
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113024639
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