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Quantitation of insertion sequence IS10 transposase gene expression by a method generally applicable to any rarely expressed gene.
We have found that IS10 transposase is synthesized in tiny amounts, about 0.15 polypeptide chain per cell per generation on average, as judged from the beta-galactosidase activity of a single chromosomal copy of a suitable transposase-lacZ gene fusion. Enzymatic activity from the fusion gene is a fa...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323169/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006072/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.6.1787 |
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