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Quantitation of insertion sequence IS10 transposase gene expression by a method generally applicable to any rarely expressed gene.

We have found that IS10 transposase is synthesized in tiny amounts, about 0.15 polypeptide chain per cell per generation on average, as judged from the beta-galactosidase activity of a single chromosomal copy of a suitable transposase-lacZ gene fusion. Enzymatic activity from the fusion gene is a fa...

詳細記述

保存先:
書誌詳細
主要な著者: Raleigh, E A, Kleckner, N
フォーマット: Artigo
言語:Inglês
出版事項: 1986
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC323169/
https://ncbi.nlm.nih.gov/pubmed/3006072
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