Quantitation of insertion sequence IS10 transposase gene expression by a method generally applicable to any rarely expressed gene.

We have found that IS10 transposase is synthesized in tiny amounts, about 0.15 polypeptide chain per cell per generation on average, as judged from the beta-galactosidase activity of a single chromosomal copy of a suitable transposase-lacZ gene fusion. Enzymatic activity from the fusion gene is a fa...

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Bibliographic Details
Main Authors: Raleigh, E A, Kleckner, N
Format: Artigo
Language:Inglês
Published: 1986
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC323169/
https://ncbi.nlm.nih.gov/pubmed/3006072
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