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Purification of microsomal signal peptidase as a complex.

We report here the purification to near homogeneity of signal peptidase from canine pancreatic microsomes. Purification was monitored using an improved post-translational assay. A 42-fold enrichment over starting membranes was achieved by selective solubilization in nonionic detergent/high-salt buff...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Evans, E A, Gilmore, R, Blobel, G
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322907/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3511473/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.581
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