Purification of microsomal signal peptidase as a complex.
We report here the purification to near homogeneity of signal peptidase from canine pancreatic microsomes. Purification was monitored using an improved post-translational assay. A 42-fold enrichment over starting membranes was achieved by selective solubilization in nonionic detergent/high-salt buff...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1986
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322907/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3511473/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.581 |
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