Reconstitution of translocation-competent membrane vesicles from detergent-solubilized dog pancreas rough microsomes.
Dog pancreas rough microsomes were solubilized in 1% octyl beta-glucoside, and membrane vesicles were reconstituted by slow 30-fold dilution with a buffer of low ionic strength. Asymmetric assembly of the membranes occurred during reconstitution since the vesicles formed contained ribosomes bound on...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298616/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602384/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.24.9931 |
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